POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73539

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all POR/Cytochrome P450 Reductase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human POR (NP_000932) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

76.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free (NBP1-47914) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free

Western Blot: POR/Cytochrome P450 Reductase Antibody (OTI3F10)Azide and BSA Free [NBP2-73539]

Western Blot: POR/Cytochrome P450 Reductase Antibody (OTI3F10)Azide and BSA Free [NBP2-73539]

Western Blot: POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free [NBP2-73539] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 Reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with a
Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free [NBP2-73539]

Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free [NBP2-73539]

Immunocytochemistry/Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free [NBP2-73539] - Staining of HT29 cells using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
Immunohistochemistry: POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free [NBP2-73539]

Immunohistochemistry: POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free [NBP2-73539]

Immunohistochemistry: POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free [NBP2-73539] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
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Applications for POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POR/Cytochrome P450 Reductase

NADPH-Cytochrome P450 Reductase (P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells. P450R is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. P450R is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450. This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. P450R is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of P450R can result in disordered steroidogenesis and Antley-Bixler syndrome.

Long Name

NADPH Cytochrome P450 Reductase

Alternate Names

CPR, CYPOR, Cytochrome P450 Reductase, P450R

Gene Symbol

POR

Additional POR/Cytochrome P450 Reductase Products

Product Documents for POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POR/Cytochrome P450 Reductase Antibody (OTI3F10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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