PDE3B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46183
![Western Blot: PDE3B Antibody [NBP1-46183]](https://resources.bio-techne.com/images/products/PDE3B-Antibody-Western-Blot-NBP1-46183-img0005.jpg "Western Blot: PDE3B Antibody [NBP1-46183]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 500 and 550 of human Phosphodiesterase 3B using the numbering given in entry NP_000913.2 (GeneID 5140).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PDE3B Antibody - BSA Free (NBP1-46183) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PDE3B Antibody - BSA Free
Western Blot: PDE3B Antibody [NBP1-46183]
Western Blot: PDE3B Antibody [NBP1-46183] - Detection of Human PDE3B by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE3B antibody NBP1-46183 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PDE3B was also immunoprecipitated by rabbit anti-PDE3B antibody NBP1-46184, which recognizes a downstream epitope. For blotting immunoprecipitated PDE3B, the blot Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for PDE3B Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PDE3B
Long Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Alternate Names
CGI-PDE B, CGIP1, CGIPDE1
Gene Symbol
PDE3B
Additional PDE3B Products
Product Documents for PDE3B Antibody - BSA Free
Product Specific Notices for PDE3B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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