PAPSS1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13730
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: QERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PAPSS1 Antibody - BSA Free (NBP2-13730) is a polyclonal antibody validated for use in IHC, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PAPSS1 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: PAPSS1 Antibody [NBP2-13730]](https://resources.bio-techne.com/images/products/PAPSS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13730-img0005.jpg "Immunocytochemistry/ Immunofluorescence: PAPSS1 Antibody [NBP2-13730]")
Immunocytochemistry/ Immunofluorescence: PAPSS1 Antibody [NBP2-13730]
Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody [NBP2-13730] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.![Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-13730]](https://resources.bio-techne.com/images/products/PAPSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-13730-img0006.jpg "Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-13730]")
Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-13730]
Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-13730] - Staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.![Simple Western: PAPSS1 Antibody [NBP2-13730]](https://resources.bio-techne.com/images/products/PAPSS1-Antibody-Simple-Western-NBP2-13730-img0008.jpg "Simple Western: PAPSS1 Antibody [NBP2-13730]")
Simple Western: PAPSS1 Antibody [NBP2-13730]
Simple Western: PAPSS1 Antibody [NBP2-13730] - Lane view shows a specific band for PAPSS1 using 200 ug/mL of HepG2 cell lysate and 1:25 antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 65 kDa. Image from an internal validation.Applications for PAPSS1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200-1:500
Simple Western
1:25
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate, separated by Size, antibody dilution of 1:25, apparent MW was 65 kDa
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate, separated by Size, antibody dilution of 1:25, apparent MW was 65 kDa
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PAPSS1
Alternate Names
3'-phosphoadenosine 5'-phosphosulfate synthase 1, ATPSK1SK 1, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, EC 2.7.1.25, PAPS synthase 1, PAPSS 1, PAPSS3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1, SK1, Sulfurylase kinase 1
Gene Symbol
PAPSS1
Additional PAPSS1 Products
Product Documents for PAPSS1 Antibody - BSA Free
Product Specific Notices for PAPSS1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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