p53 Antibody (CL2199) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43954

Novus Biologicals, part of Bio-Techne
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NBP3-43954
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BSA Free
NBP2-34495
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2199

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all p53 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P04637, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse p53 Antibody (CL2199) - Azide and BSA Free (NBP2-34495) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for p53 Antibody (CL2199) - Azide and BSA Free

p53 Antibody (CL2199) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]

Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]

Staining in A431 cell line with Anti-p53 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
p53 Antibody (CL2199) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]

Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]

Staining in MCF7 cell line with Anti-p53 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
p53 Antibody (CL2199) - Azide and BSA Free Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]

Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]

Analysis in human skin and skeletal muscle tissues using NBP3-43954 antibody. Corresponding p53 RNA-seq data are presented for the same tissues.
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Applications for p53 Antibody (CL2199) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:20

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293T, separated by Size, antibody dilution of 1:20, apparent MW was 63 kDa

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 Antibody (CL2199) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p53 Antibody (CL2199) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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