p53 Antibody (CL2199) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34495

Catalog #
Availability
Size / Price
Qty
NBP2-34495-25ul
NBP2-34495
Print Quote
Bulk Order
Conjugate
Catalog #
Alexa Fluor 350
NBP3-43954AF350
View Product
Alexa Fluor 405
NBP3-43954AF405
View Product
Alexa Fluor 488
NBP3-43954AF488
View Product
Alexa Fluor 532
NBP3-43954AF532
View Product
Alexa Fluor 594
NBP3-43954AF594
View Product
Alexa Fluor 647
NBP3-43954AF647
View Product
Alexa Fluor 700
NBP3-43954AF700
View Product
Alexa Fluor 750
NBP3-43954AF750
View Product
Alexa Fluor Plus 405
NBP3-43954AFP405
View Product
Alexa Fluor Plus 488
NBP3-43954AFP488
View Product
Alexa Fluor Plus 555
NBP3-43954AFP555
View Product
Alexa Fluor Plus 594
NBP3-43954AFP594
View Product
Alexa Fluor Plus 647
NBP3-43954AFP647
View Product
Alexa Fluor Plus 680
NBP3-43954AFP680
View Product
Allophycocyanin
NBP3-43954APC
View Product
Biotin
NBP3-43954B
View Product
CoraFluor 1
NBP3-43954CL1
View Product
DyLight 350
NBP3-43954UV
View Product
DyLight 405
NBP3-43954V
View Product
DyLight 488
NBP3-43954G
View Product
DyLight 550
NBP3-43954R
View Product
DyLight 594
NBP3-43954DL594
View Product
DyLight 650
NBP3-43954C
View Product
DyLight 680
NBP3-43954FR
View Product
DyLight 755
NBP3-43954IR
View Product
FITC
NBP3-43954F
View Product
HRP
NBP3-43954H
View Product
Janelia Fluor 525
NBP3-43954JF525
View Product
Janelia Fluor 549
NBP3-43954JF549
View Product
Janelia Fluor 646
NBP3-43954JF646
View Product
Janelia Fluor 669
NBP3-43954JF669
View Product
mFluor Violet 450 SE
NBP3-43954MFV450
View Product
mFluor Violet 500 SE
NBP3-43954MFV500
View Product
mFluor Violet 610 SE
NBP3-43954MFV610
View Product
PE
NBP3-43954PE
View Product
PerCP
NBP3-43954PCP
View Product
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence, PCR

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2199

Format

BSA Free

Concentration

1 mg/ml

View all p53 Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P04637, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for p53 Antibody (CL2199) - BSA Free

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.
Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251
View 10 more images

Applications for p53 Antibody (CL2199) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:5000

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:50

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293T, separated by Size, antibody dilution of 1:50, apparent MW was 63 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for p53 Antibody (CL2199) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for p53 Antibody (CL2199) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.