p53 Antibody (CL2199) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34495

Novus Biologicals, part of Bio-Techne
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NBP2-34495-25ul
NBP2-34495
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence, PCR

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2199

Format

BSA Free

Concentration

1 mg/ml

View all p53 Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P04637, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for p53 Antibody (CL2199) - BSA Free

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.
Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
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Applications for p53 Antibody (CL2199) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:5000

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:50

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293T, separated by Size, antibody dilution of 1:50, apparent MW was 63 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for p53 Antibody (CL2199) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p53 Antibody (CL2199) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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