p27/Kip1 Antibody (rKIP1/1356) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49591
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rKIP1/1356
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human CDKN1B protein (Uniprot: P46527)
Localization
Cytoplasm, Endosome, Nucleus
Specificity
This antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Scientific Data Images for p27/Kip1 Antibody (rKIP1/1356) - Azide and BSA Free
Western Blot: p27/Kip1 Antibody (rKIP1/1356) - Azide and BSA Free [NBP3-49591] -
Western blot analysis of 3T3 lysate using p27/Kip1 Antibody (rKIP1/1356) - Azide and BSA Free.
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (rKIP1/1356) - Azide and BSA Free [NBP3-49591] -
Formalin-fixed, paraffin-embedded human breast carcinoma stained with p27/Kip1 Antibody (rKIP1/1356) - Azide and BSA Free. Inset: PBS instead of primary antibody. Secondary negative control.Applications for p27/Kip1 Antibody (rKIP1/1356) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: p27/Kip1
Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Alternate Names
CDKN1B, Kip1
Gene Symbol
CDKN1B
Additional p27/Kip1 Products
Product Documents for p27/Kip1 Antibody (rKIP1/1356) - Azide and BSA Free
Product Specific Notices for p27/Kip1 Antibody (rKIP1/1356) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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