Occludin Antibody (CL1555)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52053

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # CL1555

Concentration

1 mg/ml

View all Occludin Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q16625, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Occludin Antibody (CL1555)

Occludin Antibody (CL1555) Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1555) [NBP3-52053]

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1555) [NBP3-52053]

Staining of CACO-2 cells showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Occludin Antibody (CL1555) Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Staining of human colon shows strong membranous positivity in glandular cells.
Occludin Antibody (CL1555) Western Blot: Occludin Antibody (CL1555) [NBP3-52053]

Western Blot: Occludin Antibody (CL1555) [NBP3-52053]

Lane 1: Marker [kDa]
Lane 2: Human cell line CACO-2
Lane 3: Human cell line U-87
View 7 more images

Applications for Occludin Antibody (CL1555)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Occludin

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Alternate Names

BLCPMG, OCLN

Gene Symbol

OCLN

Additional Occludin Products

Product Documents for Occludin Antibody (CL1555)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Occludin Antibody (CL1555)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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