Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43573
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 12H2
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing female BALB/c mice with human alpha4beta2 receptor protein purified in n-dodecyl-beta-D-maltopyranoside (DDM). (Uniprot# P17787)
Specificity
This antibody recognizes human alpha4beta2 receptor protein. The AChR responds by an extensive change in conformation after binding acetylcholine, that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Scientific Data Images
Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - Chimeric - BSA Free [NBP3-43573] -
Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - Chimeric - BSA Free [NBP3-43573] - Immunofluorescence analysis of paraformaldehyde fixed Kelly cells on coverslips stained with NBP3-43573 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43573, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.Applications
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nicotinic Acetylcholine R beta 2/CHRNB2
Long Name
Nicotinic Acetylcholine Receptor beta 2
Alternate Names
Cholinergic Receptor Nicotinic Beta 2 Subunit, CHRNB2, EFNL3, NAChRB2, Neuronal Nicotinic Acetylcholine R beta 2
Gene Symbol
CHRNB2
Additional Nicotinic Acetylcholine R beta 2/CHRNB2 Products
- All Products for Nicotinic Acetylcholine R beta 2/CHRNB2
- Nicotinic Acetylcholine R beta 2/CHRNB2 cDNA Clones
- Nicotinic Acetylcholine R beta 2/CHRNB2 ELISA Kits
- Nicotinic Acetylcholine R beta 2/CHRNB2 Lysates
- Nicotinic Acetylcholine R beta 2/CHRNB2 Primary Antibodies
- Nicotinic Acetylcholine R beta 2/CHRNB2 Proteins and Enzymes
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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