NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44219
![NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]](https://resources.bio-techne.com/images/products/nbp3-44219_mouse-nfkb2-nfkb-p100-mab-cl8266-azide-and-bsa-free-28220251312414.jpg "Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]")
![NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]](https://resources.bio-techne.com/images/products/nbp3-44219_mouse-nfkb2-nfkb-p100-mab-cl8266-azide-and-bsa-free-282202512101819.jpg "Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL8266
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of Q00653, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free (NBP2-88914) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free
![NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]](https://resources.bio-techne.com/images/products/nbp3-44219_mouse-nfkb2-nfkb-p100-mab-cl8266-azide-and-bsa-free-28220251232482.jpg "Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]")
Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]
Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.![NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]](https://resources.bio-techne.com/images/products/nbp3-44219_mouse-nfkb2-nfkb-p100-mab-cl8266-azide-and-bsa-free-28220251375691.jpg "Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]")
Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]
Staining of human skeletal muscle shows very weak nuclear positivity in myocytes as expected.![NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]](https://resources.bio-techne.com/images/products/nbp3-44219_mouse-nfkb2-nfkb-p100-mab-cl8266-azide-and-bsa-free-28220251375637.jpg "Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]")
![NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]](https://resources.bio-techne.com/images/products/nbp3-44219_mouse-nfkb2-nfkb-p100-mab-cl8266-azide-and-bsa-free-282202513355817.jpg "Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]")
![NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]](https://resources.bio-techne.com/images/products/nbp3-44219_mouse-nfkb2-nfkb-p100-mab-cl8266-azide-and-bsa-free-28220251338151.jpg "Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP3-44219]")
Applications for NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NFkB2
NFkB1 (NFkB p50) and NFkB2 (NFkB p52) are class I members of the Rel/NFkB family of transcription factors that also includes RelA, c-Rel and RelB. Rel/NFkB members regulate expression of genes that participate in immune, apoptotic and oncogenic processes. Class I members lack a C-terminal transcriptional activation segment found in class II members, but contain an RHD (Rel homology domain) that mediates DNA binding, nuclear localization and dimerization. NFkB1 and NFkB2 105 and 100 kDa precursors are proteolytically cleaved to 50 and 52 kDa active subunits, respectively. NFkB is predominantly localized in the cytoplasm as a complex with inhibitory IkB proteins and is released and translocated to the nucleus after phosphorylation of IkB.
Long Name
Nuclear Factor Kappa-B, Subunit 2
Alternate Names
LYT10, NF-kB2
Gene Symbol
NFKB2
Additional NFkB2 Products
Product Documents for NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free
Product Specific Notices for NFkB2/NFkB p100 Antibody (CL8266) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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