NF-H Antibody (rNF421)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48940
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Chicken
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rNF421 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human NEFH protein (Uniprot: P12036)
Localization
Axon, Cell projection, Cytoplasm, Cytoskeleton
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Scientific Data Images for NF-H Antibody (rNF421)
Western Blot: NF-H Antibody (rNF421) [NBP3-48940] -
Western Blot Analysis of human brain tissue lysate using NF-H Antibody (rNF421).
Flow Cytometry: NF-H Antibody (rNF421) [NBP3-48940] -
Flow Cytometric Analysis of HEK293 cells using NF-H Antibody (rNF421) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Immunohistochemistry-Paraffin: NF-H Antibody (rNF421) [NBP3-48940] -
Formalin-fixed, paraffin-embedded human Brain stained with NF-H Antibody (rNF421).Applications for NF-H Antibody (rNF421)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: NF-H
Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Long Name
Neurofilament Protein, Heavy Chain
Alternate Names
NEFH, NFH
Gene Symbol
NEFH
Additional NF-H Products
Product Documents for NF-H Antibody (rNF421)
Product Specific Notices for NF-H Antibody (rNF421)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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