Mouse/Rat Themis Alexa Fluor® 532-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6816X
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Themis
Lys122-Lys237
Accession # Q8BGW0
Lys122-Lys237
Accession # Q8BGW0
Specificity
Detects mouse Themis in direct ELISAs and mouse and rat Themis in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Themis is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse/Rat Themis Alexa Fluor® 532-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Themis
Themis (THymus-Expressed Molecule Involved in Selection; also C6orf190, Gasp and Thylex) is a 72-74 kDa member of the Themis family of proteins. It has restricted expression, being found in double negative #2-4 thymocytes, double positive thymocytes, and CD4+ plus CD8+ thymocytes and T cells. The molecule is known to constitute part of the TCR signalosome, along with LAT, Grb2 and PLC gamma1, and is required for ERK and AP-1 activation. Themis appears to contribute to the process of positive selection. In particular, it is needed to prevent cell death during the selection process. Mouse Themis is 636 amino acids (aa) in length. It contains two globular CABIT (Cys-containing, all-beta in Themis) domains (aa 1-260 and 261-520) plus an NLS (aa 555-563). There are four isoform variants. One shows a 25 aa substitution for aa 238-636, a second possesses an alternative start site at Met90 coupled to a 13 aa substitution for aa 589-636, while third and fourth variants contain 7 aa and 17 aa substitutions for aa 589-636. Over aa 122-237, mouse Themis shares 96% and 87% aa identity with rat and human Themis, respectively.
Long Name
Thymocyte-expressed Molecule Involved in Selection
Alternate Names
C6orf190, C6orf207, Gasp, SPOT, TSEPA
Gene Symbol
THEMIS
UniProt
Additional Themis Products
Product Specific Notices for Mouse/Rat Themis Alexa Fluor® 532-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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