Mouse splenocytes were stained with either (A) Rat Anti-Mouse MICL/CLEC12A Monoclonal Antibody (Catalog # MAB2950) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Goat Anti-Rat PE-conjugated secondary antibody (Catalog # F0105B) and Rat Anti-Mouse CD11c APC-conjugated Monoclonal Antibody (Catalog # FAB69501A). Cells were first gated on Rat Anti-Mouse CD8 alpha Alexa Fluor® 488-conjugated Monoclonal Antibody-positive cells (Catalog # FAB116G, not shown) . View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse MICL/CLEC12A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: CD8+ CD11c+ Mouse Splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICL/CLEC12A

MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.

Long Name

Myeloid Inhibitory C-type Lectin-like Receptor

Alternate Names

CD371, CLEC12A, CLL-1, DCAL-2

Entrez Gene IDs

160364 (Human); 232413 (Mouse)

Gene Symbol

CLEC12A

UniProt

Q504P2

Additional MICL/CLEC12A Products

Product Documents for Mouse MICL/CLEC12A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse MICL/CLEC12A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse MICL/CLEC12A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2950

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MICL/CLEC12A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse MICL/CLEC12A Antibody

Detection of MICL/CLEC12A in Mouse Splenocytes by Flow Cytometry.