Detects mouse IL-27 p28/IL-30 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-27/IL-35 EBI3 subunit, recombinant human (rh) IL-27/IL-35 EBI3 subunit, or rhIL-27 heterodimer is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Applications for Mouse IL-27 p28/IL-30 Alexa Fluor® 488-conjugated Antibody

Application

Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IL-27 p28/IL-30

Interleukin-27 p28, also known as IL-30, is a secreted 28 kDa protein that is considered a member of the IL-6/IL-12 interfamily cytokine family. p28/IL-30 is one of several alpha-chain proteins that can associate with various beta-chain proteins to form heterodimeric cytokines in these families. The alpha-chains (e.g. IL-6, IL-11, Cardiotrophin-1, CLC/CNTF, LIF, Oncostatin M, IL-23 p19, IL-27 p28/IL-30, IL-12/IL-35 p35) have a four-helix bundle structure, while the beta-chains (e.g. EBI-3, CLF-1, IL-12/IL-23 p40) resemble class 1 cytokine receptors. These cytokines utilize heteromeric cell surface receptors which contain shared as well as ligand-specific subunits. Divergent biological responses are obtained from the combinatorial association of cytokine subunits and their interaction with various combinations of receptor subunits. Complexity in this system is increased by the generation of soluble receptors and by the competition between proteins for shared subunit pairing (1-3). p28/IL-30 is expressed by macrophages and dendritic cells, and is upregulated in these cells by inflammatory stimuli (4-9). It was first described as a partner with EBI-3 in the heterodimeric cytokine IL-27 (4). IL-27 signals through a receptor complex composed of IL-27 R alpha/WSX-1/TCCR and gp130 (4, 10, 11). This interaction enhances the proinflammatory activation of naïve CD4⁺ T cells, NK cells, mast cells, and monocytes and the cytotoxic activity of CD8⁺ T cells (4, 10, 12). IL-27 also exhibits anti-inflammatory activity, including the induction of IL-10 production by naïve and memory T cells, the activation of regulatory T cells (Treg), and the suppression of Th17 cytokine secretion (13, 14). Alternatively, p28/IL-30 associates with CLF-1 to create a cytokine that triggers responses through IL‑27 R alpha, IL‑6 R alpha, and gp130 (6). Like IL-27, p28-CLF-1 heterodimers costimulate IFN-gamma production by NK cells, and induce IL-10 secretion by CD4⁺ T cells (6). In contrast to IL-27, however, p28-CLF-1 is reported to promote the differentiation of Th17 cells (6). A third mode of p28 action enables it to stimulate cells that express both IL‑6 R alpha and gp130, but lack IL-27 R alpha (11). Similar to the IL-6 system, the presence of IL-6 R alpha on the cell surface is not even required if p28/IL-30 associates with a soluble form of IL-6 R alpha. This combination can trigger trans signaling through gp130, a mechanism that has been demonstrated for complexes of IL-6 with soluble IL‑6 R alpha (11). Overexpression of p28/IL-30 in vivo interferes with humoral antibody responses and protects from IL-12 induced liver inflammation (7, 15). Mature mouse p28/IL-30 shares 70% and 89% amino acid sequence identity with human and rat p28, respectively.

Long Name

Interleukin 27 p28

Alternate Names

IL-30, IL27 p28

Entrez Gene IDs

246778 (Human); 246779 (Mouse)

Gene Symbol

IL27

UniProt

Q8K3I6

Additional IL-27 p28/IL-30 Products

Product Documents

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COA

SDS

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Product Specific Notices for Mouse IL-27 p28/IL-30 Alexa Fluor® 488-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Reviews

Mouse IL-27 p28/IL-30 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7430G

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