Perfusion fixed paraffin-embedded sections of mouse spleen and thymus
Multiplex Immunofluorescence
5 µg/mL
Perfusion fixed paraffin-embedded sections of mouse spleen
Scientific Data Examples for Mouse CD8 alpha Antibody
Multiplex Immunofluorescence
Detection of CD8a in Mouse spleen via seqIF™ staining on COMET™
CD8a was detected in perfusion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse CD8a, Monoclonal Antibody (Catalog #MAB11715) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Immunohistochemistry
Detection of CD8 alpha in Mouse Spleen.
CD8 alpha was detected in perfusion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB11715) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Detection of CD8 alpha in Mouse Thymus.
CD8 alpha was detected in perfusion fixed paraffin-embedded sections of mouse thymus using Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB11715) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator
Background: CD8
CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).
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References
Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
Hayakawa, K. et al. (1994) Science 263:1131.
MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
Rocha, B. et al. (1992) Immunol. Today 13:449.
Wang, J. and J.R. Klein (1994) Science 265:1860.
Vermec, D. et al. (1992) J. Exp. Med. 176:47.
Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
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