Mouse 5'-Nucleotidase/CD73 Fluorescein-conjugated Antibody

Detection of 5'‑Nucleotidase/CD73 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse 5'-Nucleotidase/CD73 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4488F) or (B) Rat IgG_2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Detection of 5'-Nucleotidase/CD73 by Flow Cytometry

Changes of total leukocytes, CD73+/CD39+ cells and CD73/CD39 surface density after I/R.A: Representative flow cytometry plots of digested hearts under basal conditions compared to 3 days after I/R. B: Representative flow cytometry histograms of CD73 expression (red) under basal conditions compared to 3 days after I/R. Grey histograms = fluorescence minus one control (FMO). C: Representative flow cytometry histograms of CD39 expression (blue). Grey histograms = fluorescence minus one control (FMO). D: Increase of leukocyte populations within myocardial tissue after I/R. * P<0.05 for cells/mg heart tissue under basal conditions vs. I/R. CTC = cytotoxic t-cells, THC = T-helper cells, Treg = regulatory t-cells, BC = B-cells, NKC = NK cells, Gr = Granulocytes, Mo = Monocytes, APC = Antigen-presenting cells. E: Changes of CD73+/CD39+ cells per leukocyte population after I/R. F: Changes of CD73/CD39 surface density on CD73+/CD39+ leukocytes after I/R. Analysis was done 3 days after I/R. Values are means ± SD of n = 5 experiments. n.d. = not detectable. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22514659), licensed under a CC-BY license. Not internally tested by R&D Systems.