MMP-13 Antibody (87512) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # FAB511CL1

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

MAB511

Allophycocyanin

FAB511A

Biotin

FAB511B

DyLight 350

FAB511D

DyLight 405

FAB511E

DyLight 488

FAB511K

DyLight 550

FAB511L

DyLight 594

FAB511M

DyLight 650

FAB511W

DyLight 680

FAB511Y

DyLight 755

FAB511Z

FITC

FAB511F

HRP

FAB511H

Janelia Fluor 525

FAB511JF525

Janelia Fluor 549

FAB511I

Janelia Fluor 646

FAB511J

Janelia Fluor 669

FAB511JF669

mFluor Violet 450 SE

FAB511MFV450

mFluor Violet 500 SE

FAB511MFV500

mFluor Violet 610 SE

FAB511MFV610

PE

FAB511P

PerCP

FAB511C

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation, Immunoaffinity Purification

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # 87512

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MMP-13 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452

Specificity

Detects human MMP-13 in direct ELISAs and Western blots. Detects both pro and active forms of human MMP-13 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for MMP-13 Antibody (87512) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for MMP-13 Antibody (87512) [CoraFluor™ 1]

Application

Recommended Usage

Immunoaffinity Purification

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: MMP-13

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Gene Symbol

MMP13

Additional MMP-13 Products

Product Documents for MMP-13 Antibody (87512) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for MMP-13 Antibody (87512) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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