MED4 Antibody (24GB40)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50498
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB40
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human MED4 (Uniprot: Q9NPJ6)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MED4 Antibody (24GB40)
Immunocytochemistry/ Immunofluorescence: MED4 Antibody (24GB40)[NBP3-50498] -
Immunocytochemical staining of HepG2 cells with MED4 antibody (1:1,000). Nuclei were stained blue with DAPI; MED4 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: MED4 Antibody (24GB40)[NBP3-50498] -
Flow cytometric analysis of MED4expression in HepG2 cells using MED4 antibody (1:2,000). Green, isotype control; red, Mediator complex subunit 4.
Western Blot: MED4 Antibody (24GB40)[NBP3-50498] -
Western blotting analysis using MED4 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for MED4 Antibody (24GB40)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MED4
Long Name
Mediator Complex Subunit 4
Alternate Names
ARC36, DRIP36, HSPC126, TRAP36, VDRIP
Gene Symbol
MED4
Additional MED4 Products
Product Documents for MED4 Antibody (24GB40)
Product Specific Notices for MED4 Antibody (24GB40)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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