MAT1/2A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-94162
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat, Bovine, Mouse (Negative), Primate, Zebrafish
Cited:
Mouse
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide made to an internal portion of the human MAT2A protein (within residues 100-200). [Swiss-Prot# P31153]
Reactivity Notes
Orangutan. Does not react with mouse.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MAT1/2A Antibody - BSA Free
Western Blot: MAT1/2A AntibodyBSA Free [NB110-94162]
Western Blot: MAT1/2A Antibody [NB110-94162] - Detection of MAT2A in HepG2 whole cell lysates using NB110-94162.Simple Western: MAT1/2A AntibodyBSA Free [NB110-94162]
Simple Western: MAT1/2A Antibody [NB110-94162] - Simple Western lane view shows a specific band for MAT1/2 A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Western Blot: MAT1/2A Antibody - BSA Free [NB110-94162] -
C1 metabolic pathway and characterization of R6/2 mice. A Folate, Met and BH4 cycles in plants and animals and their associated metabolism. Red lines stand for mammal specific, green lines stand for plant specific while black lines stand for both. All enzymes with protein levels examined by immunoblotting are marked in red. B mHtt protein aggregates in cortex and striatum regions were detected with anti-Htt antibody (mEM48) in 4-week-old male R6/2 and NCAR mice. C, D Quantification analysis of immunoblotting results of GTPCH, DHFR, QDPR, MS, MAT1/2A, AHCY, MTHFR, TPH2, TH, nNOS and ChAT (n = 7). The band intensity of each protein from western blotting D was normalized with gamma-tubulin on the same blot. The ratio was further calculated against NCAR whose relative expression level was set as 1. All data plotted are the average (n = 7) +/- SD. Only one representative western blotting of gamma-tubulin is shown. Original blots of above proteins before cropping are presented in Fig. S11. E Contents of BH4 and BH2, and their ratio in brain tissues and plasma (n = 4, average +/- SD). *p < 0.05; **p < 0.01. ***p < 0.001. Abbreviations used for enzymes: AHCYS-adenosylhomocysteine hydrolase, ChAT choline acetyltransferase, DHFR dihydrofolate reductase, GTPCH GTP cyclohydrolase I, MAT1/2A methionine adenosyltransferase, MS methionine synthase, MTHFR methylene-tetrahydrofolate reductase, nNOS neuronal nitric oxide synthase, QDPR quinoid dihydropteridine reductase, TH tyrosine hydroxylase (Tyr), TPH2 tryptophan hydroxylase Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36251090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for MAT1/2A Antibody - BSA Free
Application
Recommended Usage
Simple Western
1:10
Western Blot
2 ug/ml
Application Notes
This MAT1/2A antibody is useful for Western blot, where a band is seen at approx. 43 kDa.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 74 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 74 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 review rated 5 using NB110-94162 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MAT1/2A
Alternate Names
AdoMet synthase 2, AMS2, EC 2.5.1.6, MAT 2, MATA2SAMS2adoMet synthetase 2, MATII, MAT-II, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, methionine adenosyltransferase II, alpha, S-adenosylmethionine synthase isoform type-2, S-adenosylmethionine synthetase isoform type-2
Gene Symbol
MAT2A
Additional MAT1/2A Products
Product Documents for MAT1/2A Antibody - BSA Free
Product Specific Notices for MAT1/2A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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