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MafF Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56792

Novus Biologicals, part of Bio-Techne
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NBP2-56792-25ul
NBP2-56792

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LSSKALKIKRELSENTPHLSDEALMGLSVREL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MafF Antibody - BSA Free (NBP2-56792) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MafF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MafF Antibody [NBP2-56792]

Immunocytochemistry/ Immunofluorescence: MafF Antibody [NBP2-56792]

Immunocytochemistry/Immunofluorescence: MafF Antibody [NBP2-56792] - Staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.
MafF Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: MafF Antibody - BSA Free [NBP2-56792]

Chromatin Immunoprecipitation-exo-Seq: MafF Antibody - BSA Free [NBP2-56792]

ChIP-Exo-Seq composite graph for Anti-MAFF (NBP2-56792) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for MafF Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MafF

The protein encoded by the MAFF gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog F

Alternate Names

hMafF, transcription factor MafF, U-Maf, v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F, v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein F, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)

Gene Symbol

MAFF

Additional MafF Products

Product Documents for MafF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MafF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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