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Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80120

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Bacteria

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # p6007

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant Listeria monocytogenes p60.

Reactivity Notes

Listeria monocytogenes

Specificity

Recognizes L. monocytogenes p60 protein. Does not cross-react with other Listeria species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free

Western Blot: Listeria monocytogenes p60 Antibody (p6007)Azide and BSA Free [NBP2-80120]

Western Blot: Listeria monocytogenes p60 Antibody (p6007)Azide and BSA Free [NBP2-80120]

Western Blot: Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free [NBP2-80120] - Analysis using Listeria monocytogenes p60, mAb (P6007) (Prod. No. NBP2-80120) at 1:5000 dilution.
Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free

Western Blot: Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free [NBP2-80120] -

Type I IFN suppresses Lm virulence factor degradation in phagosomes.a Schematic of experimental protocol detailing methods to obtain secreted and bacterial lysate fractions from BMDMs infected with indicated Lm strains. Representative images (b) and quantification (c) of ActA and InlB on the bacteria surface measured by western blot in BMDMs infected with indicated Lm strains 4 h p.i. Cell wall-associated protein p60 used as loading control. **P = 0.002, ****P < 0.0001. Representative images (d) and quantification (e) of LLO measured by western blot in BMDMs infected with indicated Lm strains over 4 h. *P < 0.0295, **P < 0.0039. Data shown are means +/- standard deviation (s.d.) for at least n = 3 independent experiments. P value was calculated using two-way ANOVA. ns not significant, P > 0.1301. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34404769), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000 - 1:10000

Western Blot

1:2000 - 1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:34404769).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2um-filtered solution in PBS, pH 7.4.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Listeria monocytogenes p60

Alternate Names

IAP p60 from Listeria Monocytogenes, Invasion-associated Protein p60, Invasion-associated Protein p60 from Listeria Monocytogenes, invasion-associated-protein, p60 protein

Additional Listeria monocytogenes p60 Products

Product Documents for Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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