IgG4 Fc Antibody (RM217) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62127B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-62127B-0.05mg
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Sandwich ELISA Capture, Sandwich ELISA Detection

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM217 expressed in HEK293

Concentration

1 mg/ml

View all IgG4 Fc Antibodies »

Product Specifications

Immunogen

Peptide corresponding to the Fc region of Human IgG4

Specificity

This antibody reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG4 Fc Antibody (RM217) [Biotin]

IgG4 Fc Antibody (RM217) [Biotin]

ELISA: IgG4 Fc Antibody (RM217) [Biotin] [NBP2-62127B] -

ELISA: IgG4 Fc Antibody (RM217) [Biotin] [NBP2-62127B] - Sandwich ELISA using NBP2-62127B as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.
IgG4 Fc Antibody (RM217) [Biotin]

Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] -

Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.
IgG4 Fc Antibody (RM217) [Biotin]

ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] -

ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] - A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Applications for IgG4 Fc Antibody (RM217) [Biotin]

Application
Recommended Usage

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Product Documents for IgG4 Fc Antibody (RM217) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IgG4 Fc Antibody (RM217) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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