HuR/ELAVL1 Antibody (9Q6L3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15820
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9Q6L3 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717). MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Knockout (KO) Validated Rabbit HuR/ELAVL1 Antibody (9Q6L3) (NBP3-15820) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HuR/ELAVL1 Antibody (9Q6L3)
![Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]](https://resources.bio-techne.com/images/products/HuR-ELAVL1-Antibody-9Q6L3-Western-Blot-NBP3-15820-img0008.jpg "Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]")
Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]
Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] - Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (NBP3-15820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Knockout Validated: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]](https://resources.bio-techne.com/images/products/HuR-ELAVL1-Antibody-9Q6L3-Knockout-Validated-NBP3-15820-img0001.jpg "Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]")
Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]
Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] - Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (NBP3-15820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] -
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] -Human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Applications for HuR/ELAVL1 Antibody (9Q6L3)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:100 - 1:1000
Immunohistochemistry
1:200 - 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:1000 - 1:6000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HuR/ELAVL1
Alternate Names
ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R), ELAV-like protein 1, embryonic lethal, abnormal vision, drosophila, homolog-like 1, Hu antigen R, Hua, Hu-antigen R, HuR, HURELAV1, MelG
Gene Symbol
ELAVL1
Additional HuR/ELAVL1 Products
Product Documents for HuR/ELAVL1 Antibody (9Q6L3)
Product Specific Notices for HuR/ELAVL1 Antibody (9Q6L3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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