Human VAP-1/AOC3 Antibody

Detection of VAP-1/AOC3 by Western Blot

Biophysical and functional characterization of endometrial pericytes and EnSCs. (A) Immunohistochemistry showing VAP-1 expression in luteal-phase human endometrium, hematoxylin was used to stain the nuclei. Staining indicates VAP-1 reactivity in the vasculature and spiral arteries, arrow pointing to spiral artery. Scale bar: 200 μm. (B) VAP-1 expression was determined at protein level in freshly isolated SUSD2+ pericytes and SUSD2− EnSCs from three independent endometrial biopsies by Western blot analysis and normalized to beta-actin and relative intensity determined by densitometry; Student's t-test; *P < 0.05. (C) Expression of ELN (left panel), CNN1 (middle panel) and MYH11 (right panel) transcripts was determined by RT-qPCR in freshly isolated SUSD2+ pericytes and SUSD2− EnSCs from four independent endometrial biopsies; Student's t-test; *P < 0.05; **P < 0.01. (D) Representative graphs showing proliferation and migration SUSD2+ pericytes and SUSD2− EnSCs monitored in real-time using the xCELLigence system for the indicated time-points. (E) freshly isolated SUSD2+ pericytes and SUSD2− EnSCs were embedded into collagen at a density of 1 × 106 cells per gel. Single cell contraction force was measured using the depth-sensing nanoindentation system. Data represent mean ± SEM of four biological repeat experiments. Student's t-test; **P < 0.01; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33537312), licensed under a CC-BY license. Not internally tested by R&D Systems.