Detects human Trpysin 1/PRSS1 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) Trypsin 3, recombinant human (rh) Trypsin 2, or rhTrypsin 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Trypsin 1/PRSS1 Antibody

Detection of Human Trypsin 1/PRSS1 by Western Blot

Protein expression levels of (A) PRSS1 and (B) SPINK1 in AP patients before and after treatment. Patients were divided into three groups based on the severity of their AP; mild, modest and severe. Out of a total of 50 AP patients that underwent ITCM therapy, 26 exhibited mild AP (light grey), 13 exhibited modest AP (medium grey) and 11 patients were diagnosed with severe AP (dark grey). Following ITCM treatment, 29 patients exhibited excellent therapeutic results (26 mild, two modest and one severe), 17 patients exhibited an effective outcome (11 modest and six severe) and four severe patients showed an ineffective response. Data are represented as the mean ± standard deviation of three independent experiments. *P<0.01, vs. value before treatment. AP, acute pancreatitis; ITCM, integrated traditional Chinese medicine. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25667658), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Trypsin 1/PRSS1 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Trypsin 1/PRSS1 (Catalog # 3848-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Trypsin 1/PRSS1 (Catalog # 3848-SE)
under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB3848 in the following applications:

Immunocytochemistry (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin 1/PRSS1

Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen (1). Constituting approximately two‑thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro‑enzymes (2). Mutations in the PRSS1 gene can cause hereditary pancreatitis (3).

References

Emi, M. et al. (1986) Gene 41:305.
Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1483, Academic Press, San Diego.
Teich, N. et al. (2006) Hum. Mutat. 27:721.

Alternate Names

PRSS1, PTRYI, TRY4, Trygn16, Trypsinogen 1

Entrez Gene IDs

5644 (Human); 114228 (Mouse); 24691 (Rat)

Gene Symbol

PRSS1

UniProt

P07477

Additional Trypsin 1/PRSS1 Products

Product Documents for Human Trypsin 1/PRSS1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Trypsin 1/PRSS1 Antibody

For research use only

Citations
Reviews

Human Trypsin 1/PRSS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3848

Key Product Details

Species Reactivity

Validated:

Cited:

Applications