TFF3 was detected in immersion fixed paraffin-embedded sections of human colon using 25 µg/mL Human TFF3 Monoclonal Antibody (Catalog # MAB4407) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of goblet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Trefoil Factor 3 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

1 µg/mL
Sample: Recombinant Human TFF3
under non-reducing conditions only

Reviewed Applications

Read 5 reviews rated 4.4 using MAB4407 in the following applications:

ELISA (1 Review)
Immunohistochemistry (1 Review)
Microarray (1 Review)
Microarrays (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF3

Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF3 is secreted by mucus-producing goblet cells in the large and small intestines and lower respiratory tract. It is mainly active as a disulfide-linked homodimer. The mature 59 aa, 6.5 kDa human TFF3 contains one trefoil structure formed by intramolecular disulfides and shows 76% aa identity with mouse TFF3.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Entrez Gene IDs

7033 (Human); 21786 (Mouse); 25563 (Rat)

Gene Symbol

TFF3

UniProt

Q07654

Additional TFF3 Products

Product Documents for Human Trefoil Factor 3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Trefoil Factor 3 Antibody

For research use only

Citations
Reviews

Human Trefoil Factor 3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4407

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all TFF3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Trefoil Factor 3 Antibody

TFF3 in Human Colon.