Human Trappin-2/Elafin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1747
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Immunoprecipitation
Cited:
Immunohistochemistry-Frozen, Bioassay, ELISA Detection, ELISA Development, Immunodepletion
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trappin‑2/Elafin
Ala23-Gln117
Accession # P19957
Ala23-Gln117
Accession # P19957
Specificity
Detects human Trappin‑2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM-2, and human Neutrophil Elastase is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Trappin-2/Elafin Antibody
Neutralization of Trappin‑2/ Elafin Activity by Human Trappin‑2/Elafin Antibody.
Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) activity is measured in the presence of Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747-PI) that has been pre-incubated with increasing concentrations of Goat Anti-Human Trappin-2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747). The ND50 is typically 15.6 µg/mL.
Human Trappin-2 / Elafin ELISA Standard Curve
Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI) was serially diluted and captured by Goat Anti-Human Trappin-2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Trappin-2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Human Trappin-2 / Elafin ELISA Standard Curve
Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI) was serially diluted and captured by Goat Anti-Human Trappin-2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Trappin-2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Trappin-2/Elafin Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI)
Sample: Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI)
Neutralization
Measured by its ability to neutralize Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747-PI) inhibition of Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) cleavage of the fluorogenic peptide substrate MeOSuc-AAPV-Amc (100 µM). The Neutralization Dose (ND50) is typically 15.6 µg/mL.
Human Trappin-2/Elafin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trappin-2/Elafin
Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72‑117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally. The recombinant human Trappin-2 corresponds to the full‑length form (residues 23‑117), which migrates as two protein bands under SDS-PAGE due to an unidentified mechanism. In addition to its ability to inhibit human neutrophil elastase, it can also be used as a substrate for transglutaminases.
References
- Schalkwijk, J. et al. (1999) Biochem. J. 340:569.
Long Name
Elastase Inhibitor, Transglutaminase Substrate, Serine Protease
Alternate Names
Elafin, ESI, PI3, SKALP, Trappin2
Entrez Gene IDs
5266 (Human)
Gene Symbol
PI3
UniProt
Additional Trappin-2/Elafin Products
Product Documents for Human Trappin-2/Elafin Antibody
Product Specific Notices for Human Trappin-2/Elafin Antibody
For research use only
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