Human TIMP-2 Antibody

Detection of Human TIMP‑2 by Western Blot.

Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated).This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TIMP-2 in Human Ovarian Cancer Tissue.

TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of k7k9qrz3b3wvetd0cdxv.jpg by Western Blot

Expression of matrix remodeling proteins (MMP). (A) Immunoblots showing MMPs and TIMP‐2 expression (representative blots are shown). (B) Quantification of protein expression [A/B: n = 2 for MMP7 and MT1‐MMP, n = 3 for tissue inhibitor of MMPs (TIMP)1, n = 4 for MMP‐9 and n = 5 for MMP‐2; SEM, t‐test, P < 0.05, **P < 0.01]. (C) MMP activity in cell supernatants determined by gelatin‐zymography (n = 3, one representative zymogram is shown). (D) RNA expression of MMPs and TIMP‐2 in PAR, FR and FR/TK cells determined by RNASeq analysis (n = 3, ANOVA; n.s., not significant, *P < 0.05, ****P < 0.0001; FR, fast running shuttle cells; PAR, parental shuttle cells). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38115217), licensed under a CC-BY license. Not internally tested by R&D Systems.