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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 976128

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TDAG8/GPR65
Met1-Glu337
Accession # Q8IYL9

Specificity

Detects human TDAG8/GPR65 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human TDAG8/GPR65 Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TDAG8/GPR65

Human G Protein-Coupled Receptor 65 (GPR65), also known as T-Cell Death-Associated Gene 8 Protein (TDAG8) and Psychosine Receptor, is a 337 aminoacids proton-sensing GPCR primarily expressed in lymphoid tissues and is uniquely expressed in Th17 cells; mice deficient in GPR65 are resistant to autoimmunity. GPR65 senses pH by protonation of histidine residues on its extracellular domain. Many pH-sensing G protein-coupled receptors have emerged as potential therapeutic agents in conditions that involve the response to acidotic stress.

Long Name

G Protein-coupled Receptor 65

Alternate Names

GPR65, Psychosine Receptor

Entrez Gene IDs

8477 (Human); 14744 (Mouse); 299242 (Rat)

Gene Symbol

GPR65

UniProt

Additional TDAG8/GPR65 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TDAG8/GPR65 Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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