Species Reactivity:

Human

Immunogen:

E. coli-derived recombinant human SGK1
Met1-Leu431
Accession # O00141

Applications:

Immunohistochemistry, Western Blot

Label:

Alexa Fluor 405 (Excitation=405 nm, Emission=421 nm)

Specificity:

Detects human SGK1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SGK2 and rhSGK3 is observed.

View Full Technical Details for Human SGK1 Alexa Fluor® 405-conjugated Antibody

Technical Data
Citations / Reviews

Applications for Human SGK1 Alexa Fluor® 405-conjugated Antibody

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

=

Mass (in vial)

÷

Desired Reconstitution Concentration

Background: SGK1

Serum- and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases. In addition to serum and glucocorticoids, insulin, IGF-I, osmotic shock, and mineralocorticoids have been demonstrated to activate SGK1. Expressed at low levels, SGK1 appears to function as a regulator of epithelial ion transport. Sustained high levels of SGK1 activation have been implicated in hypertension and diabetic nephropathy.

Item	Value
Long Name	Serum/Glucocorticoid Regulated Kinase 1
Entrez Gene IDs	6446 (Human); 20393 (Mouse); 29517 (Rat)
Alternate Names	EC 2.7.11, EC 2.7.11.1, SGK, SGK1, Serum/glucocorticoid-regulated kinase 1, serine/threonine protein kinase SGK, serine/threonine-protein kinase Sgk1, serum/glucocorticoid regulated kinase, serum/glucocorticoid regulated kinase 1

Customer Reviews for Human SGK1 Alexa Fluor® 405-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human SGK1 Alexa Fluor® 405-conjugated Antibody and earn rewards!

Have you used Human SGK1 Alexa Fluor® 405-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Product Documents for Human SGK1 Alexa Fluor® 405-conjugated Antibody

Product Datasheets

Download Product Datasheets

Human SGK1 Alexa Fluor® 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3200V

Key Product Details