Human Semaphorin 4A Alexa Fluor™ Plus 680-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4694AFP680

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FAB4694AFP680-100UG
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 741531

View all Semaphorin 4A Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4A

Specificity

Detects human Semaphorin 4A in direct ELISAs. In direct ELISAs, 50%-100% cross-reactivity with recombinant human (rh) Semaphorin 4C and rhSemaphorin 4G is observed, and less than 5% cross-reactivity with rhSemaphorin 4B, 4D, and recombinant mouse Semaphorin 4A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Semaphorin 4A

Semaphorin 4A (Sema4A, previously semB) is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems (1). The 761 amino acid (aa) human Sema4A precursor contains a 32 aa signal sequence, a 651 aa extracellular domain (ECD) containing sema, PSI and C2-type immunoglobulin domains, a 21 aa transmembrane domain, and a 57 aa cytoplasmic domain with two Ser/Thr phosphorylation sites (2). Human Sema4A ECD shares 87%, 87%, 86% and 85% aa identity with mouse, rat, bovine and canine Sema4A, respectively, and shares 32-37% aa identity with other human Sema4 family members. Of six reported splice variants with 723, 629, 370, 321, 236 and 220 aa, five lack the N-terminus and/or portions of the sema domain, and three lack the transmembrane and cytoplasmic domains in the C-terminus (3). Sema4A was first described as a molecule that enhances T cell activation and interacts with TIM-2 (T cell immunoglobulin and mucin domain-2 (4). Mice with targeted disruption of Sema4A show defects in dendritic cell-mediated T cell priming and Th1 responses (5). Roles for Sema4A have also been identified in the brain, the endothelium and the eye. It mediates hippocampal neuron growth cone collapse in vitro through interaction of the sema domain with Plexin-B1 (6). Interaction of Sema4A with endothelial cell Plexin-D1 causes opposition to the angiogenic, proliferative, chemotactic and integrin-mediated adhesive actions of VEGF (7). The retina of Sema4A-/- mice shows severe degeneration, and mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans (8, 9).

References

  1. Kumanogoh, A. et al. (2003) J. Cell Sci. 116:3463.
  2. Swissprot Accession # Q9H3S1.
  3. Entrez Accession # CAI15528, CAI15529, CAI15531, CAI15532, CAI15533 and EAW52993.
  4. Kumanogoh, A. et al. (2002) Nature 419:629.
  5. Kumanogoh, A. et al. (2005) Immunity 22:305.
  6. Yukawa, K. et al. (2005) Int. J. Mol. Med. 16:115.
  7. Toyofuku, T. et al. (2007) EMBO J. 26:1373.
  8. Rice, D.S. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:2767.
  9. Abid, A. et al. (2007) J. Med. Genet. 43:378.

Alternate Names

CORD10, SEMA4A, SEMAB, SEMB

Entrez Gene IDs

64218 (Human); 20351 (Mouse); 310630 (Rat)

Gene Symbol

SEMA4A

UniProt

Q9H3S1 (Human)

Additional Semaphorin 4A Products

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Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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