Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Sheep IgG

View all SALM2/LRFN1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SALM2/LRFN1

Specificity

Detects human SALM2/LRFN1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SALM3 and rhSALM4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human SALM2/LRFN1 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SALM2/LRFN1

Synaptic adhesion-like molecule 2 (SALM2; also leucine-rich repeat and fibronectin type-III domain-containing protein 1 (Lrfn1) is a 105 kDa member of the Lrfn family of type I transmembrane glycoproteins (1‑2). Human SALM2 is synthesized as a 771 amino acid (aa) precursor that contains a 31 aa signal sequence, a 505 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 214 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 3). In addition, there are two potential sites for N-linked glycosylation. The C-terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1‑3, but is absent in SALMs 4 and 5 (4). Mature human SALM2 shares 96% and 95% aa sequence identity with mature mouse and rat SALM2, respectively. In the developing mouse embryo, the temporal expression profile blot revealed a general increment of expression around E10.5 for SALM2. Northern blot analysis showed that in rats, SALM2 is strongly expressed in the adult brain and is also present in the adult testis (2). SALM2 localizes to both axons and dendrites (4). In addition, it co-localizes with both pre- and post-synaptic proteins at excitatory synapses in mature neurons (2, 4). SALM2, like the other SALMs, promotes neurite outgrowth (4). In particular, SALM2 promotes an increase in the number of primary processes compared to controls and SALMs 1 and 5 (4). In addition, SALM2 is an important regulator of the differentiation of excitatory synapses (2). SALM2 may also be involved in synaptic maintenance and other cellular interactions (4).

Long Name

Synaptic Adhesion-like Molecule 2/Leucine-rich Repeat and Fibronectin Type III Domain Containing 1

Alternate Names

LRFN1

Entrez Gene IDs

57622 (Human); 365222 (Rat)

Gene Symbol

LRFN1

Additional SALM2/LRFN1 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SALM2/LRFN1 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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