Human ROR alpha/NR1F1 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC8924P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 784652
Product Specifications
Immunogen
E. coli-derived recombinant human ROR alpha/NR1F1
Arg47-His163
Accession # P35398
Arg47-His163
Accession # P35398
Specificity
Detects human ROR alpha/NR1F1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human ROR alpha/NR1F1 PE-conjugated Antibody
Detection of ROR alpha/NR1F1 in Th17 Differentiated Human PBMCs by Flow Cytometry.
Th17 differentiated human peripheral blood mononuclear cells (PBMCs, panel A) or resting PBMCs (panel B) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and Mouse Anti-Human RORa/NR1F1 PE-conjugated Monoclonal Antibody (Catalog # IC8924P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of ROR alpha/NR1F1 in Jurkat cells by Flow Cytometry
Jurkat cells were stained with Mouse Anti-Human ROR alpha/NR1F1 PE-conjugated Monoclonal Antibody (Catalog # IC8924P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human ROR alpha/NR1F1 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Th17 differentiated human peripheral blood mononuclear cells (PBMCs) or Jurkat human acute T cell leukemia cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Sample: Th17 differentiated human peripheral blood mononuclear cells (PBMCs) or Jurkat human acute T cell leukemia cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR alpha/NR1F1
Long Name
Retinoic Acid-Related Orphan Receptor alpha
Alternate Names
NR1F1, RORA, RZRA
Entrez Gene IDs
6095 (Human)
Gene Symbol
RORA
UniProt
Additional ROR alpha/NR1F1 Products
Product Documents for Human ROR alpha/NR1F1 PE-conjugated Antibody
Product Specific Notices for Human ROR alpha/NR1F1 PE-conjugated Antibody
For research use only
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