Human/Rat Vinculin Alexa Fluor® 532-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6896X
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Vinculin
Lys1020-Gln1134
Accession # P18206
Lys1020-Gln1134
Accession # P18206
Specificity
Detects recombinant human Vinculin in direct ELISAs and human and rat Vinculin in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human/Rat Vinculin Alexa Fluor® 532-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Vinculin
VCL (Vinculin; from the Latin vinculum denoting a union or connection) is a 115-120 kDa cytoplasmic member of the vinculin/ alpha-catenin family of proteins. The VCL gene is ubiquitously expressed and generates a series of globular domains whose N-and C-terminal globular regions self-associate to generate an inactive molecule. When at least two ligands bind to the molecule, it opens up, allowing for the formation of multiple complexes that impact both signaling and cell structure. Among other things, VCL is known to regulate the expression of E-cadherin, and to connect actin to integrins via talin. Human VCL is 1134 amino acids (aa) in length, and at this size, is commonly known as metavinculin. It contains an 835 aa N-terminal globular head that binds talin, beta- and alpha-catenin, followed by a Pro-rich linker segment (aa 836-878) and a 27-29 kDa C-terminal tail that binds lipids plus paxillin and PKC alpha (aa 879-1134). At least seven utilized phosphorylation sites exist. There are three potential splice variants. One shows a deletion of aa 916-983 and is called vinculin. A second contains an alternative start site at Met74, and again possesses a deletion of aa 916-983. A third shows a 34 aa substitution for aa 262‑1134. Over the range of aa used as the immunogen for this reagent (aa 1020-1134 in metavinculin; 952-1066 in vinculin), human and mouse VCL are identical in aa sequence.
Long Name
Metavinculin
Alternate Names
MVCL, VCL
Gene Symbol
VCL
UniProt
Additional Vinculin Products
Product Specific Notices for Human/Rat Vinculin Alexa Fluor® 532-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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