Detects human Presenilin‑1 C-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Presenilin‑1 N-Terminal Fragment (aa 1‑80), Presenilin‑2 C‑Terminal Fragment (aa 322‑388), and rhPresenilin‑2N‑Terminal Fragment (aa 1‑80) is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Presenilin-1 C-Terminal Fragment Antibody

Detection of Human Presenilin‑1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Presenilin-1 C-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Presenilin-1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Human Presenilin‑1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Presenilin-1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Presenilin-1 C-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Presenilin-1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Presenilin-1 C-Terminal Fragment Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Knockout Validated

Presenilin‑1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Presenilin‑1 knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, and HEK293T human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Presenilin-1

Presenilin-1 (PS-1) and Presenilin-2 (PS-2) are integral membrane proteins that contain multiple transmembrane domains. Both are predominantly located within the endoplasmic reticulum (ER) and early Golgi apparatus. They are primarily expressed in neurons and are ubiquitously expressed in the brain. PS-1 and PS-2 are proteolytically cleaved to generate an N-terminal fragment (NTF) and a C-terminal fragment (CTF). Mutations in the gene for Presenilin may play an important role in the pathology of Alzheimer's disease.

Alternate Names

Presenilin1, PSEN1, S182

Entrez Gene IDs

5663 (Human)

Gene Symbol

PSEN1

Additional Presenilin-1 Products

Product Documents for Human Presenilin-1 C-Terminal Fragment Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Presenilin-1 C-Terminal Fragment Antibody

For research use only

Citations
Reviews

Human Presenilin-1 C-Terminal Fragment Antibody

R&D Systems, part of Bio-Techne | Catalog # AF166

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications