Western blot shows lysates of MCF-7 human breast cancer cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/ml Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes and 100 ng/ml Recombinant Human PDGF-BB (Catalog # 220-BB) for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-PRAS40 (T246) Monoclonal Antibody (Catalog # MAB68901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PRAS40 (T246) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-PRAS40 (T246) Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line and NIH‑3T3 mouse embryonic fibroblast cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1) and Recombinant Human PDGF‑BB (Catalog # 220-BB)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRAS40

PRAS40 (Proline-rich AKT1 substrate 1), also known as Akt1S1 and p39, is a 40-42 kDa cytoplasmic phosphoprotein that lacks generally recognized structural motifs. It is widely expressed and is considered to be key regulator of mTORC1 (mTOR plus Raptor and G betaL), a complex through which Akt signals into the cell. Through phosphorylation, mTORC1 activity is upregulated by PRAS40. In particular, nonphosphorylated PRAS40 binds to and serves as a negative regulator of mTORC1 activity. Upon Insulin signaling, PRAS40 is phosphorylated on Thr246, Ser221 and Ser183. This causes it to bind 14-3-3 and results in its dissociation from mTORC1, freeing up mTOR to regulate (positively or negatively) protein synthesis. Human PRAS40 is 256 amino acids (aa) in length. It contains one extended Pro-rich region (aa 35-96) plus at least nine utilized Ser/Thr phosphorylation sites. There is one alternative start site at Met131. Over aa 119-256, human PRAS40 shares 93% aa sequence identity with mouse PRAS40.

Long Name

40 kDa Proline-rich Akt1 Substrate

Alternate Names

AKT1S1, Lobe

Entrez Gene IDs

84335 (Human); 67605 (Mouse); 292887 (Rat)

Gene Symbol

AKT1S1

UniProt

Q96B36 (Human)

Additional PRAS40 Products

Product Documents for Human Phospho-PRAS40 (T246) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Phospho-PRAS40 (T246) Antibody

For research use only

Citations
Reviews

Human Phospho-PRAS40 (T246) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB68901

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all PRAS40 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-PRAS40 (T246) Antibody

Detection of Human and Mouse Phospho-PRAS40 (T246) by Western Blot.