Human Phospho-GCN2 (T899) Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7605G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human GCN2 T899 site
Specificity
Detects human GCN2 when phosphorylated at T899 in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: GCN2
GCN2 (General Control Non-derepressible 2-like protein; also eIF2a kinase 4) is a 190-200 kDa member of the GCN2 subfamily, Ser/Thr kinase family, protein kinase superfamily of proteins It is widely expressed, being found in skeletal muscle cells, fibroblasts, hepatocytes and neurons. GCN2 is a key regulator of metabolism under nutrient deprivation. In particular, when essential amino acids (aa) are limiting, tRNAs exist that are unbound to amino acids. These “uncharged” tRNAs will bind to GCN2, activating the molecule. Through GCN2-mediated phosphorylation of eIF2a, promotes GCN4 synthesis, with subsequent activation of amino acid synthesizing enzymes, and a downregulation of lipid storage. Human GCN2 is 1649 aa in length. It possesses one RWD domain (aa 25-137), two protein kinase domains (aa 296-539 and 590-1001) and an extended His-RS-like region (aa 1021-1492). There are at least three utilized Ser/Thr phosphorylation sites. Two isoform variants exist. One shows a deletion of aa 774-801, while another contains a nine aa substitution for aa 608-1649. Over aa 22-139, human GCN2 shares 91% aa sequence identity with mouse GCN2.
Long Name
General Control Nonrepressed 2-like Protein
Alternate Names
EIF2AK4
Gene Symbol
EIF2AK4
Additional GCN2 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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