Human peripheral blood mononuclear cells (PBMCs) were treated with 5 μg/ml PHA for 5 days then stained with Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody FAB100P) and either (A) Rabbit Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10545) or (B) Rabbit IgG Isotype Control (MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody F0111). View our protocol for Staining Membrane-associated Proteins.

Applications for Human OX40 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human PBMC

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OX40

OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response (1-3). Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that contains a cysteine-rich region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (4). The ECD of human OX40 shares 63% sequence identity with the ECD of mouse and rat OX40. OX40 is up-regulated on CD4⁺ and CD8⁺ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7 (5, 6). OX40 Ligand is primarily expressed on antigen presenting cells (5). OX40 Ligand engagement of OX40 on activated CD4⁺ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells (3, 7-10). OX40 is constitutively expressed on Tregs and enhances the sensitivity of Tregs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells’ immunosuppressive activity on effector T cells (11-14). OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease (6, 15, 16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (17, 18).

References

Hori, T. (2006) Int. J. Hematol. 83:17.
Latza, U. et al. (1994) Eur. J. Immunol. 24:677.
Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695.
Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230.
Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
Xiao, X. et al. (2008) J. Immunol. 181:3193.
So, T. and M. Croft (2007) J. Immunol. 179:1427.
Mousavi, S.F. et al. (2008) J. Immunol. 181:5990.
Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902.
Griseri, T. et al. (2010) J. Exp. Med. 207:699.
Xiao, X. et al. (2012) J. Immunol. 188:892.
Vu, M.D. et al. (2007) Blood 110:2501.
Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
Xiao, X. et al. (2012) Nat. Immunol. 13:981.
Nakano, M. et al. (2010) Cardiovasc. Res. 88:539.
Ishii, N. et al. (2010) Adv. Immunol. 105:63.
Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757

Alternate Names

ACT-135, CD134, Ly-70, TNFRSF4, Txgp1, TXGP1L

Entrez Gene IDs

7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)

Gene Symbol

TNFRSF4

UniProt

P43489

Additional OX40 Products

Product Documents for Human OX40 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human OX40 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human OX40 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10545

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all OX40 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human OX40 Antibody

Detection of OX40/TNFRSF4 in Human PBMCs by Flow Cytometry.