HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB4728, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Neuromedin B R/NMBR in HEK293 Human Cell Line Transfected with Human Neuromedin B R/NMBR and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) Neuromedin B R/NMBR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB4728) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Neuromedin BR/NMBR Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Neuromedin B R/NMBR and eGFP

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HT-29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuromedin BR/NMBR

Neuromedin B Receptor (NMBR) is an 80 kDa, 390 amino acid (aa) G-protein coupled 7-transmembrane glycoprotein receptor for bombesin-like peptides, binding Neuromedin B with high affinity and GRP (gastrin releasing peptide) with lower affinity. Neuromedin B R expression in the olfactory and central thalamic regions of the brain plays a role in thermoregulation. It has also been shown to be mitogenic in colonic epithelium. Expression in the pituitary gland is important for regulation of the pituitary-thyroid axis. Within extracellular domains, human Neuromedin B R shares 86% and 82% aa identity with mouse and rat Neuromedin B R, respectively.

Long Name

Neuromedin B Receptor

Alternate Names

Neuromedin B R, NMBR

Entrez Gene IDs

4829 (Human); 18101 (Mouse); 25264 (Rat)

Gene Symbol

NMBR

UniProt

AAB27330

Additional Neuromedin BR/NMBR Products

Product Documents for Human Neuromedin BR/NMBR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Neuromedin BR/NMBR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Neuromedin BR/NMBR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4728

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Neuromedin BR/NMBR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Neuromedin BR/NMBR Antibody

Detection of Neuromedin B R/ NMBR in HT‑29 Human Cell Line by Flow Cytometry.