Species Reactivity:

Human

Immunogen:

Mouse myeloma cell line NS0-derived recombinant human Nephrin
Gln23-Thr1029
Accession # O60500

Applications:

Immunohistochemistry, Western Blot

Label:

Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)

Specificity:

Detects human Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Nephrin is observed.

View Full Technical Details for Human Nephrin Alexa Fluor® 647-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Reconstitution Calculator

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Background: Nephrin

Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa’s 1056-1095 are deleted, eliminating the transmembrane region. Over aa 23-1029, human Nephrin shares 84% and 89% aa sequence identity with mouse and canine Nephrin, respectively.

Item	Value
Entrez Gene IDs	4868 (Human); 54631 (Mouse); 64563 (Rat)
Alternate Names	CNF, NPHNCNF, NPHS1, Nephrin, Renal glomerulus-specific cell adhesion receptor, nephrosis 1, congenital, Finnish type (nephrin)

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Human Nephrin Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4269R

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