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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 584825

Product Specifications

Immunogen

E. coli-derived recombinant human NEDD9/CASL
Lys153-Asn366
Accession # Q14511

Specificity

Detects human NEDD9/CASL in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NEDD4 or rhNEDD8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human NEDD9/CASL Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NEDD9/CASL

NEDD9 (also HEF1 and CASL) is a 93 kDa (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin beta1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Tyr-rich SH2-binding region (aa 90-350), one Ser-rich four alpha-helix bundle protein interaction site (aa 350‑650), and an HLH motif that mediates Cas protein dimerization (aa 710-760). NEDD9 usually runs at 105 or 115 kDa in SDS-PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55 kDa fragment. One isoform shows a deletion of aa 155-188, while two others show 20 or 7 aa substitutions for aa 155-834 or 1-153, respectively. Over aa 153-366, human and mouse NEDD9 share 85% aa identity.

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9

Alternate Names

CAS2, CASL, HEF1, p105

Entrez Gene IDs

4739 (Human); 18003 (Mouse); 291044 (Rat)

Gene Symbol

NEDD9

UniProt

Additional NEDD9/CASL Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human NEDD9/CASL Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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