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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human NAALADase-like 1/NAALADL1
Ala32-Leu740
Accession # NP_005459

Specificity

Detects human NAALADase-like 1/NAALADL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) NAALADase I, recombinant mouse NAALADase II, and rhNAALADase II is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NAALADase-like 1/NAALADL1

NAALADase-like1, also known as NAALADL1, (N-acetylated alpha-linked acidic dipeptidase like 1; also Ileal dipeptidylpeptidase) is a 100-115 kDa member of the peptidase M28 family of enzymes. It is found in the brush border of Ileal epithelium and demonstrates serine protease activity. Human NAALADL1 is a type II transmembrane glycoprotein that is 740 amino acids (aa) in length. It contains an extended extracellular domain (aa 29‑740) that shows a protease-associated region (aa 109‑335) with key catalytic residues at Ser617, Asp657 and His687. Multiple splice variants exist. Two show deletions of aa 161‑201 and 296‑330, respectively, while a third shows a 51 aa insertion after Pro359. Three show 18 to 42 aa substitutions after Pro502, with premature truncations after Ile522, Thr543 and Leu579. A final isoform shows a 17 aa substitution for aa 619‑740. Over aa 32‑740, human NAALADL1 shares 81% aa identity with mouse NAALADL1.

Long Name

N-Acetylated Alpha-Linked Acidic Dipeptidase-like 1

Alternate Names

ileal dipeptidylpeptidase, NAALADase L, NAALADase like 1, NAALADase-like 1, NAALADL1

Entrez Gene IDs

10004 (Human); 381204 (Mouse); 83568 (Rat)

Gene Symbol

NAALADL1

UniProt

Additional NAALADase-like 1/NAALADL1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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