Human/Mouse SIN3A Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6115T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SIN3A
Tyr1187-Pro1273
Accession # Q96ST3
Tyr1187-Pro1273
Accession # Q96ST3
Specificity
Detects human and mouse SIN3A in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human/Mouse SIN3A Alexa Fluor® 594-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: SIN3A
SIN3A (Switch insensitive-3 A) is a nuclear, 140‑150 kDa member of the SIN3 family of co-repressors. SIN3A plays a key role in gene repression. Normally, genes are not expressed when chromatin is "closed", or condensed, precluding transcriptional activators from assembling on gene promoters. This state is achieved through histone deacetylation and DNA methylation, and SIN3A serves as a scaffold for multiple proteins, including deacetylases (HDAC1 and 2). Human SIN3A is 1273 amino acids (aa) in length. It contains three PAH (paired amphipathic helix) repeats (aa 141‑187, 322‑381 and 477‑523) and multiple phosphoserines and acetylated lysines. There are three potential splice variants. The first shows a 33 aa substtution for aa 124‑1273, while the second and third show eight aa substitutions for aa 337‑352, coupled to either a deletion of aa 1009‑1066, or a two aa substitution for aa 1008‑1065. Over aa 1187‑1273, human and mouse SIN3A show 100% identity in aa sequence.
Long Name
SIN3 Homolog A
Alternate Names
DKFZp434K2235, FLJ90319, Histone deacetylase complex subunit Sin3a, KIAA0700, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator (yeast), SIN3 homolog A, transcriptional regulator (yeast), Transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A
Gene Symbol
SIN3A
UniProt
Additional SIN3A Products
Product Specific Notices for Human/Mouse SIN3A Alexa Fluor® 594-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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