Human/Mouse/Rat TRRAP Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7365V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TRRAP
Asn3610-Leu3859, predicted
Accession # Q9Y4A5
Asn3610-Leu3859, predicted
Accession # Q9Y4A5
Specificity
Detects human, mouse and rat TRRAP in Western blots. Detects recombinant human TRRAP in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human/Mouse/Rat TRRAP Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: TRRAP
TRRAP (Transformation/transcription domain-associated protein; also PAF350/400 and STAF40) is a 400-450 kDa member of the TRA1 subfamily, PI3/PI4 kinase family, ATM superfamily of molecules. It is a ubiquitously expressed nuclear protein that functions as a promoter of gene transcription. In particular, the activity of the E2F transcription factor family is regulated by the degree of histone acetylation. Deacetylation inhibits E2F-mediated transcription, while acetylation promotes gene transcription. TRRAP promotes histone acetylation by serving as a positive modulator/cofactor for GCN5 acetyltransferase activity. TRRAP also acts independently of GCN5. It serves as a recruiter for Skp1, a ubiquitin ligase that ubiquitates chromatin-bound beta-catenin, leading to a downregulation of Wnt-signaling activity. Human TRRAP is 3859 amino acids (aa) in length. It contains one Pro-rich region (aa 485-526), an NLS (aa 2047-2062), two FAT domains (aa 2704-3275 and 3827-3859) and a nonenzymatic PI3/PI4 kinase domain (aa 3528-3826). There are multiple potential isoform variants. They show various combinations of a deletion involving aa 1493-1510, an Ala substitution for aa 3001-3012, a 119 aa substitution for aa 3784-3859, and an 18 aa insertion after Asp3287. Over aa 3610-3859, human TRRAP shares 99% aa identity with mouse TRRAP.
Long Name
Transformation/Transcription Domain-associated Protein
Alternate Names
PAF400, STAF40, TR-AP, Tra1
Entrez Gene IDs
8295 (Human)
Gene Symbol
TRRAP
UniProt
Additional TRRAP Products
Product Specific Notices for Human/Mouse/Rat TRRAP Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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