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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 883411

Product Specifications

Immunogen

E. coli-derived recombinant human NKX2.2
Met1-Lys128
Accession # O95096

Specificity

Detects human NKX2.2 in ELISA and Western Blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human/Mouse/Rat NKX2.2 Alexa Fluor® 350-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NKX2.2

NKX2.2, also known as NKX2-2 or NKX2B, is an approximately 30 kDa, 273 amino acid homeobox nuclear transcription factor of the NKX2 protein family. It specifies differentiation of pancreatic islet cells such as alpha, beta, PP, and epsilon lineages.  It also functions in developmental neuronal patterning of the ventral spinal cord in response to graded Sonic Hedgehog gradients and contributes pathfinding of commissural neurons. Within the region used as an immunogen, human NKX2.2 shares 96% and 97% amino acid sequence identity with mouse and rat NKX2.2, respectively.

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Entrez Gene IDs

4821 (Human); 18088 (Mouse); 366214 (Rat)

Gene Symbol

NKX2-2

UniProt

Additional NKX2.2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat NKX2.2 Alexa Fluor® 350-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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