Western blot shows lysates of CCD-1070Sk human foreskin fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRB7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4856) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRB7 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GRB7 in Human Liver Cancer Tissue.

GRB7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Sheep Anti-Human/Mouse/Rat GRB7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4856) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat GRB7 Antibody

Application

Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue

Western Blot

0.5 µg/mL
Sample: CCD-1070Sk human foreskin fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRB7

GRB7 (Growth factor receptor-bound protein 7) mediates the coupling of multiple cell surface receptors to downstream signaling pathways in the regulation of various cellular functions, such as proliferation, apoptosis, and cell migration. GRB7 protein consists of several distinctive structural features, including an amino-terminal proline-rich region, a central GM region (for Grb and Mig), and a carboxyl-terminal SH2 domain. GRB7 is phosphorylated by focal adhesion kinase at cell adhesion sites.

Long Name

Growth Factor Receptor-Bound Protein 7

Alternate Names

B47

Entrez Gene IDs

2886 (Human); 14786 (Mouse); 84427 (Rat)

Gene Symbol

GRB7

UniProt

Q14451

Additional GRB7 Products

Product Documents for Human/Mouse/Rat GRB7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat GRB7 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat GRB7 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4856

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GRB7 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat GRB7 Antibody

Detection of Human/Mouse/Rat GRB7 by Western Blot.