Gelsolin/GSN was detected in immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma using Mouse Anti-Human Gelsolin/GSN, pan Monoclonal Antibody (Catalog # MAB8170) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of Human, Mouse, and Rat Gelsolin/GSN by Western Blot.

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Gelsolin/GSN by Simple Western^TM.

Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685), Exosome Standards (U-87 MG) (NBP2-49844) and SK-Mel-28 human malignant melanoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Gelsolin/GSN at approximately 90 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Gelsolin/GSN in Human Kidney.

Gelsolin/GSN was detected in formalin fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and distal convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Shows Human Gelsolin/GSN Specificity Using Knockout Cell Line.

Western blot shows lysates of U2OS human osteosarcoma cell line and Gelsolin/GSN knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Gelsolin/GSN at approximately 80 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of hbahwou3etm9tvbh4pxm.jpg by Immunohistochemistry

GSN expression in muscle pericytes and fibroblasts. (A) Immunohistochemical analysis of GSN and CD13 (pericyte marker) expression in mouse TA muscle. (B) Quantification revealed GSN expression in approximately 90% of CD13+ pericytes in TA muscle. n = 4 mice. Data were shown as mean ± SD. (C) Immunohistochemical analysis of GSN and vimentin (fibroblast marker) expression in mouse TA muscle. (D) Quantification revealed GSN expression in approximately 14% of vimentin+ fibroblasts in TA muscle. n = 4 mice. Data were shown as mean ± SD. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-025-14225-3), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Gelsolin/GSN by Immunohistochemistry

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Applications for Human/Mouse/Rat Gelsolin/GSN Antibody

Application

Recommended Usage

COMET

Immunohistochemistry

8-25 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human kidney

Knockout Validated

Gelsolin/GSN is specifically detected in the parental U2OS cell line, but is not detectable in knockout U2OS cell line.

Multiplex Immunofluorescence

20 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma

Simple Western

5-20 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685), Exosome Standards (U-87 MG) (Catalog # NBP2-49844) and SK‑Mel‑28 human malignant melanoma cell line

Western Blot

0.5 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line

Reviewed Applications

Read 1 review rated 5 using MAB8170 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gelsolin/GSN

Gelsolin, also known as GSN, actin-depolymerizing factor/ADF, AGEL and Brevin, is a 90-95 kDa member of the villin/gelsolin family. Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm. Cytoplasmic Gelsolin lacks 51 N-terminal amino acids (aa) present in the secreted protein. Gelsolin was identified by its ability to sever actin filaments in the presence of submicromolar calcium, and plays a role in ciliogenesis. Defects in Gelsolin cause amyloidosis type 5, a hereditary disease characterized by gelsolin amyloid deposition. Full-length human Gelsolin is 782 aa and shares 93% aa identity with mouse and rat Gelsolin.

Alternate Names

ADF, AGEL, Brevin, GSN

Entrez Gene IDs

2934 (Human); 227753 (Mouse); 296654 (Rat)

Gene Symbol

GSN

UniProt

P06396 (Human)

Additional Gelsolin/GSN Products

Product Documents for Human/Mouse/Rat Gelsolin/GSN Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat Gelsolin/GSN Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat Gelsolin/GSN Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8170

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications