Human/Mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6664
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human beta-1,4-Glucuronyltransferase 1/B4GAT1
Asp43-Cys415
Accession # O43505
Asp43-Cys415
Accession # O43505
Specificity
Detects human and mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 in Western blot. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) B4GalT1 and rhB3GNT 2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody
Detection of Human and Mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 by Western Blot.
Western blot shows lysates of human muscle tissue and mouse muscle tissue. PVDF Membrane was probed with 1 µg/mL of Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for beta-1,4-Glucuronyltransferase 1/B4GAT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human/Mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human muscle tissue and mouse muscle tissue
Sample: Human muscle tissue and mouse muscle tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-1,4-Glucuronyltransferase 1/B4GAT1
B3GNT1 ( beta-1,3-N-acetylglucosaminyltransferase 1; also iGnT) is a 50 kD member of the beta3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451 amino acid (aa) type II transmembrane protein. It contains an eight aa N-terminal cytoplasmic segment, an extended 28 aa transmembrane region (aa 9-36), and a 379 aa luminal domain (aa 37-415). Over aa 37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.
Long Name
beta-1,4-Glucuronyltransferase 1
Alternate Names
B3GNT1, iGAT, iGNT, MDDGA13
Gene Symbol
B4GAT1
UniProt
Additional beta-1,4-Glucuronyltransferase 1/B4GAT1 Products
Product Documents for Human/Mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody
Product Specific Notices for Human/Mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody
For research use only
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