Human MMP-19 Alexa Fluor® 532-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6790X
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-19
Leu229-Tyr508
Accession # Q99542
Leu229-Tyr508
Accession # Q99542
Specificity
Detects human MMP-19 in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human MMP-19 Alexa Fluor® 532-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: MMP-19
MMP-19 (Matrix metalloprotease 19; also MMP-18 and MMP RASI) is a 55-59 kDa member of the peptidase M10A family of enzymes. It is widely expressed, being secreted by stratum basale keratinocytes, smooth muscle cells, epiphysial cartilage chondrocytes and monocytes/macrophages. MMP-19 has multiple substrates, including components of the basement membrane (type IV collagen; laminin; nidogen), fibronectin, aggrecan plus COMP, and IGFBP3, this latter cleavage resulting in the release of active IGF-I. Studies involving MMP-19 demonstrate an antiangiogenic function. This is attributable to the processing of plasminogen, generating angiostatin-like molecules, and the creation of an environment that promotes the ECM retention of soluble VEGF. Human proMMP-19 is 490 amino acids (aa) in length. It contains an autocleavable 9 kDa propeptide (aa 19-97) plus a 411 aa mature region (aa 98-508). The mature region contains a Zn catalytic region (aa 103-256) plus four hemopexin-like domains (aa 293-508). There are three additional potential isoforms. One shows an 88 aa substitution for aa 300-508, a second contains a 12 aa substitution for aa 1-298, while a third possesses an alternative start site at Met80. Over aa 229-508, human MMP-19 shares 76% aa identity with mouse MMP-19.
Long Name
Matrix Metalloproteinase 19
Alternate Names
MMP-18, MMP19, RASI-1
Gene Symbol
MMP19
UniProt
Additional MMP-19 Products
Product Specific Notices for Human MMP-19 Alexa Fluor® 532-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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