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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Miz-1
Met1-Ala188
Accession # Q13105

Specificity

Detects endogenous human Miz-1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Miz-1/ZBTB17 Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Miz-1/ZBTB17

Miz-1 (Myc-interacting zinc finger protein 1; also ZBTB17) is an 88 kDa, DNA-binding protein that belongs to the Kruppel C2H2-type zinc finger protein family. Human Miz-1 is 803 amino acids (aa) in length, and contains a POZ domain (aa 1 - 104), two Myc-interaction segments (aa 269 - 308 & 637 - 718), and 12 C2H2-type zinc finger motifs (aa 306 - 637); also, there is one alternate splice form that shows an eight aa substitution for Thr769. Miz-1 induces cell arrest at G1, an effect mediated by its activation of the gene coding for p15INK4b. This effect is blocked by Myc, which displaces transcriptional coactivators bound to Miz-1. Notably, although the downregulation of Miz-1 may contribute to Myc-induced cell transformation, the deactivation of Miz-1 is absolutely essential for Myc-induced apoptosis. Over aa 1‑188, human Miz-1 is 85% aa identical to mouse Miz-1.

Long Name

Myc-interacting Zinc Finger Protein-1

Alternate Names

mZ13, ZBTB17, Zfp100, ZNF151

Entrez Gene IDs

7709 (Human); 22642 (Mouse); 313666 (Rat)

Gene Symbol

ZBTB17

UniProt

Additional Miz-1/ZBTB17 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Miz-1/ZBTB17 Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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