Human Lysosomal Pro-X Carboxypeptidase/PRCP Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7164AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Lysosomal Pro‑X Carboxypeptidase/PRCP
Specificity
Detects human Lysosomal Pro‑X Carboxypeptidase/PRCP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Lysosomal Pro-X Carboxypeptidase/PRCP
PRCP (Prolylcarboxypeptidase; also PCP, lysosomal Pro-X carboxypeptidase and lysosomal carboxypeptidase C) is a lysosomal 57-62 kDa glycoprotein member of the S28 family of proteases. It is expressed by a number of cells, including fibroblasts, macrophages, and endothelial cells. PRCP cleaves a variety of single C‑terminal amino acids (aa) adjacent to a Pro residue, and is known to act on, and inactivate, peptides such as prekallikrein, alpha-MSH and angiotensin II plus III. Human PRCP is apparently synthesized as a prepropeptide that contains a signal sequence (aa 1-21), a short prosequence (aa 22-45) and a 451 aa mature region (aa 46-496). The mature region demonstrates a Ser-carboxypeptidase domain (aa 55-477) that, in itself, possesses an SKS domain that caps the enzyme's catalytic site. The molecule is known to be mannosylated, and to form noncovalent homodimers. There is one splice variant that contains a 21 aa insertion after Lys56. Over aa 1-496, human and mouse PRCP share 77% aa sequence identity.
Alternate Names
Angiotensinase C, HUMPCP, Prolylcarboxypeptidase
Gene Symbol
PRCP
UniProt
Additional Lysosomal Pro-X Carboxypeptidase/PRCP Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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